A high-throughput screening method for determining the substrate scope of nitrilases.
نویسندگان
چکیده
Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 13 شماره
صفحات -
تاریخ انتشار 2015